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	Provedor de dados BABT (2) Genet. Mol. Biol. (1) Autor Oliveira,Naila Francis Paulo de (3) Arruda,Isabela Tatiana Sales de (1) Bezerra,Stephane Flaviane de Oliveira (1) Costa,Ludimila de Araújo (1) Dolder,Mary Anne Heidi (1) Freitas,Priscylla Alves Nascimento de (1) Genari,Selma Candelária (1) Persuhn,Darlene Camati (1) Mais... Palavra-chave DNA methylation (2) Epigenetic (2) Aging (1) Blood (1) DNA repair (1) In vitro (1) Lymphocytes (1) MTHFR (1) MTHFR C677T (1) Microscopy (1) Morphology (1) Oral epithelial cells (1) Percoll (1) Polymorphism (1) Telomerase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2015 (1) 2013 (1) 2010 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Age-related Changes in DNA Methylation Status of hTERT Gene Promoter of Oral Epithelial Cells BABT Bezerra,Stephane Flaviane de Oliveira; Costa,Ludimila de Araújo; Freitas,Priscylla Alves Nascimento de; Oliveira,Naila Francis Paulo de. The purpose of this study was to investigate the effect of aging on the DNA methylation status of two genes involved in tumorigenesis (telomerase gene hTERT and DNA repair gene- MLH1) and one in metabolism (methylenetetrahydrofolate reductase gene- MTHFR) in oral epithelial cells. DNA methylation analysis was performed by Methylation Sensitive Restriction Enzymes (MSRE) of healthy oral epithelial cells of child (6-10 years, n=21), young (20-25 years, n=19) and elderly (over 60 years, n=25). The results for the hTERT gene showed significant variation in the methylation frequency at CpG dinucleotides among the groups (p=0.0001), with the methylated condition more frequently in children and young people. In relation to MLH1 and MTHFR, no differences were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Epigenetic; DNA methylation; Aging; DNA repair; Telomerase; MTHFR. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100082 Light microscope observation of circulating human lymphocytes cultured in vitro BABT Oliveira,Naila Francis Paulo de; Dolder,Mary Anne Heidi; Genari,Selma Candelária. The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes. Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; In vitro; Lymphocytes; Microscopy; Morphology; Percoll. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013 The MTHFR C677T polymorphism and global DNA methylation in oral epithelial cells Genet. Mol. Biol. Arruda,Isabela Tatiana Sales de; Persuhn,Darlene Camati; Oliveira,Naila Francis Paulo de. DNA methylation is mediated by DNA methyltransferases (DNMTs) that add a methyl group to the 5'-carbon of cytosine. The enzyme methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the rate-limiting step of the cycle involving the methyl donor S-adenosyl-L-methionine (SAM). The MTHFR C677T polymorphism results in a thermolabile enzyme with reduced activity that is predicted to influence the DNA methylation status. In this study, we investigated the impact of the MTHFR C677T polymorphism on the global DNA methylation of oral epithelial cells obtained from 54 healthy subjects. There were no significant differences in global DNA methylation among the MTHFR CC, CT and TT genotypes... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA methylation; Epigenetic; MTHFR C677T; Oral epithelial cells; Polymorphism. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400004 Registros recuperados: 3 Primeira ... 1 ... Última

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